Method enabling gene expression studies of pathogens in a complex food matrix.
نویسندگان
چکیده
We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 77 23 شماره
صفحات -
تاریخ انتشار 2011